Biotinated probe containing a long-terminal repeat hybridized to a mouse colon tumor and normal tissue

Science  23 Dec 1983:
Vol. 222, Issue 4630, pp. 1339-1341
DOI: 10.1126/science.6689218


The cloned complementary DNA pMCT-1, which contains an intracisternal A particle long-terminal repeat, is more highly expressed in a mouse colon tumor than in the normal mouse colon. In situ hybridization of biotin-substituted pMCT-1 to fixed frozen sections shows that expression of pMCT-1 is seen throughout the tumor and is highly heterogeneous on a cellular basis, while expression is undetectable in any cell in the normal colonic mucosa.