Appearance of a new nucleosomal protein during differentiation of human leukemia (HL-60) cells

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Science  30 Mar 1984:
Vol. 223, Issue 4643, pp. 1420-1423
DOI: 10.1126/science.6583846


A 60-kilodalton protein was identified in chromatin digested by micrococcal nuclease during retinoic acid-induced differentiation of human leukemia (HL-60) cells to mature-like granulocytes. The protein was not detected in a retinoic acid-resistant variant of the HL-60 cell line treated with retinoic acid, in HL-60 cells induced with dimethyl sulfoxide, or in normal human granulocytes. This protein may have an important role in the regulation of retinoic acid-induced leukemic cell differentiation.