Transient Expression of Homologous Genes in Drosophila Cells

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Science  01 Jun 1984:
Vol. 224, Issue 4652, pp. 1004-1006
DOI: 10.1126/science.224.4652.1004


A cloned Drosophila heat shock protein 22 gene was transfected into two independently established Drosophila cell lines. Each line carried a different heat shock protein 22 allele, distinguishable by electrophoresis of the protein. The transfected gene was not expressed at 25°C but could be induced at 36°C. In one line, two heat shock protein 22 electromorphs were synthesized.