Isolation, characterization, and chromosome assignment of mouse N-ras gene from carcinogen-induced thymic lymphoma

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Science  07 Sep 1984:
Vol. 225, Issue 4666, pp. 1041-1043
DOI: 10.1126/science.6089339


Treatment of mice with the carcinogen N-methylnitrosourea results in the development of thymic lymphomas with frequent involvement of the N-ras oncogene. The activated mouse N-ras gene was isolated from one of these lymphomas and, by transformation in concert with restriction digestion, a map of the gene was prepared and its approximate boundaries were determined. By means of somatic cell hybrids the normal N-ras gene was found to be unlinked to other members of the ras gene family.