Inhibitors of lysosomal enzymes: accumulation of lipofuscin-like dense bodies in the brain

See allHide authors and affiliations

Science  23 Nov 1984:
Vol. 226, Issue 4677, pp. 985-987
DOI: 10.1126/science.6505679


Injections of leupeptin (a thiol proteinase inhibitor) or chloroquine (a general lysosomal enzyme inhibitor) into the brains of young rats induced the formation of lysosome-associated granular aggregates (dense bodies) which closely resembled the ceroid-lipofuscin that accumulates in certain disease states and during aging. The dense material increased in a dose- and time-dependent fashion and was differentially distributed across brain regions and cell types. These observations provide clues to the origins of ceroid-lipofuscin and suggest means for studying the consequences of its accumulation.