Cloned mycoplasma ribosomal RNA genes for the detection of mycoplasma contamination in tissue cultures

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Science  07 Dec 1984:
Vol. 226, Issue 4679, pp. 1211-1213
DOI: 10.1126/science.6505688


A cloned fragment of the mycoplasma ribosomal RNA operon was used as a molecular probe for the detection of mycoplasmas in cell cultures. According to the conditions of hybridization, the probe can detect prokaryotes in general or mycoplasmas specifically.