Homologous recombination catalyzed by mammalian cell extracts in vitro

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Science  07 Dec 1984:
Vol. 226, Issue 4679, pp. 1213-1215
DOI: 10.1126/science.6334360


An assay was developed to detect recombination events taking place in an in vitro reaction. Extracts of cultured mouse preB lymphocytes were found to catalyze homologous recombination between substrate DNA molecules but not site-specific recombination between cloned mouse immunoglobulin D and J genes. Addition of deoxyribonucleoside triphosphates increased the frequency of homologous recombination. This recombination activity was not observed in two differentiated lymphocyte cell lines.