Reports

A single recombinant plasmid expressing two major outer surface proteins of the Lyme disease spirochete

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Science  08 Feb 1985:
Vol. 227, Issue 4687, pp. 645-646
DOI: 10.1126/science.3969554

Abstract

A gene bank of DNA from the Lyme disease spirochete was constructed in the plasmid pBR322. Plasmid pTRH32, a recombinant that in Escherichia coli expresses the two major outer surface proteins of the Lyme disease spirochete, was identified. One of the recombinant products, designated OspA, represents a surface protein that appears to be common to all Lyme disease spirochetes, whereas the other recombinant product, designated OspB, represents a more variable surface protein. This recombinant plasmid provides a foundation for future studies on the epidemiology and pathogenesis of Lyme disease as well as on the genetic organization of the etiologic agent.

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