Asymmetric illumination contrast: a method of image formation for video light microscopy

Science  15 Feb 1985:
Vol. 227, Issue 4688, pp. 766-768
DOI: 10.1126/science.3969565


Images with high resolution and exceptionally broad gray scale can be obtained by the application of video contrast enhancement to an optimized procedure for imaging transparent objects with oblique rays of illumination. This technique is simple to set up. A conventional microscope with a light source whose position can be adjusted and a video camera with controls for gain and black level are the only essential components. Features such as high resolution, optical sectioning, control of contrast, and operation under low light intensity make this technique preferable, in several instances, to currently used video microscopy techniques.