Construction and recovery of viable retroviral genomes carrying a bacterial suppressor transfer RNA gene

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Science  19 Apr 1985:
Vol. 228, Issue 4697, pp. 329-332
DOI: 10.1126/science.2984770


The integration of retroviral genomes into cellular DNA can induce mutations by altering the expression of nearby cellular genes and can serve to identify the gene affected. The construction of a retrovirus that stably carries a suppressor transfer RNA gene from Escherichia coli has allowed facile recovery of the viral genome in vectors marked with amber mutations. This virus can be used for rapid isolation of cellular sequences at the site of proviral insertion.