DNA elements are asymmetrically joined during the site-specific recombination of kappa immunoglobulin genes

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Science  10 May 1985:
Vol. 228, Issue 4700, pp. 677-685
DOI: 10.1126/science.3158075


Immunoglobulin K genes are constructed during lymphocyte differentiation by the joining of two DNA elements, VK and JK, to form both a VKJK coding unit and a reciprocal recombination product. The two products formed in single VK-to-JK joining events can be directly isolated through the use of a retrovirally introduced recombination substrate. The structural analysis of a number of recombinants and the derivation of secondary recombination products define some of the basic features of the mechanism of immunoglobulin gene assembly.