A role for glycosylation of the alpha subunit in transduction of biological signal in glycoprotein hormones

Science  05 Jul 1985:
Vol. 229, Issue 4708, pp. 65-67
DOI: 10.1126/science.2990039


The biological properties of recombinants of glycoprotein hormones in which the alpha and beta subunits were differentially deglycosylated have been investigated. Specific deglycosylation of the alpha subunit generated a recombinant that had more receptor-binding activity but did not produce hormone response in the target cells. The deglycosylated alpha + beta recombinant was also an antagonist of the action of the native hormone. Thus, the carbohydrates in the alpha subunit play a dominant role in the transduction of the hormone signal into the cell.