Synthesis of Chlorophyllide b from Protochlorophyllide in Chlamydomonas reinhardtii y-1

Science  25 Oct 1985:
Vol. 230, Issue 4724, pp. 450-453
DOI: 10.1126/science.230.4724.450


In cells of Chlamydomonas reinhardtii y-1 kept in the dark, 1,7-phenanthroline stimulated the conversion of protochlorophyllide to chlorophyllide b. A membrane fraction was obtained from degreened cells that was active in this conversion only when phenanthroline was present. Untreated cells excreted protochlorophyllide, which was used as substrate for this in vitro reaction. This system may provide a clue to how chlorophyllide b is synthesized in plant cells.