Transposon tagging to detect a latent virus in Myxococcus xanthus

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Science  01 Nov 1985:
Vol. 230, Issue 4725, pp. 541-543
DOI: 10.1126/science.2996138


Transposon mutagenesis of the bacterium Myxococcus xanthus with the transposon Tn5 revealed a special class of bacterial mutants that transduced the transposon through culture supernatant fluids. Virus-like particles copurified with transducing activity. Transposon tagging for detecting these virus-like particles may be generally useful in isolating endogenous viral agents capable of transferring genetic information between cells.