Evolution of bunyaviruses by genome reassortment in dually infected mosquitoes (Aedes triseriatus)

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Science  01 Nov 1985:
Vol. 230, Issue 4725, pp. 548-550
DOI: 10.1126/science.4048949


Aedes triseriatus mosquitoes became dually infected after ingesting two mutants of LaCrosse (LAC) virus simultaneously or after ingesting, by interrupted feeding, the two viruses sequentially within a 2-day period. After 2 weeks of incubation, approximately 25 percent of the vectors contained new virus genotypes as the result of RNA segment reassortment. New viruses were transmitted when the mosquitoes fed on mice. Viruses ingested more than 2 days after the initial infecting virus did not cause superinfection of the mosquito vectors.