Isolation of a mammalian sequence capable of conferring cell cycle regulation to a heterologous gene

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Science  29 Nov 1985:
Vol. 230, Issue 4729, pp. 1061-1063
DOI: 10.1126/science.4059922


A hybrid gene containing the 5' sequence of a hamster histone H3 gene and the coding sequence of the bacterial neomycin-resistance gene (neo) was constructed. Upon transfection into the hamster fibroblast cell line K12, the hybrid gene exhibited cell cycle-dependent regulation, as evidenced by the maximal accumulation of the neo transcripts during synthesis of DNA in the cell cycle. In addition, cells arrested in the prereplicative phase, as a consequence of the K12 temperature-sensitive mutation, produced significantly less neo messenger RNA.