Research Articles

Separation of DNA binding from the transcription-activating function of a eukaryotic regulatory protein

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Science  14 Feb 1986:
Vol. 231, Issue 4739, pp. 699-704
DOI: 10.1126/science.3080805

Abstract

The yeast GAL4 protein (881 amino acids) binds to specific DNA sites upstream of target genes and activates transcription. Derivatives of this protein bearing as few as 74 amino terminal residues bind to these sites but fail to activate transcription. When appropriately positioned in front of a gene these derivatives act as repressors. These and related findings support the idea that GAL4 activates transcription by touching other DNA-bound proteins.