Endonucleolytic activity that cleaves immunoglobulin recombination sequences

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Science  07 Mar 1986:
Vol. 231, Issue 4742, pp. 1141-1145
DOI: 10.1126/science.3003919


An endonucleolytic activity has been identified in nuclear extracts of chick embryo bursa and mouse fetal liver cells. The activity introduces a double-strand cut in the vicinity of the recombination site of immunoglobulin joining gene segments. The cleavage occurs at the dinucleotide pair TG-AC. This activity is a good candidate for the putative endonuclease involved in recombination of the immunoglobulin variable, diversity, and joining regions. It is distinct from the endonuclease activities previously reported by others.