Plant glutamine synthetase complements a glnA mutation in Escherichia coli

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Science  06 Jun 1986:
Vol. 232, Issue 4755, pp. 1242-1244
DOI: 10.1126/science.2871626


A glutamine synthetase gene from alfalfa (Medicago sativa) has been expressed in Escherichia coli after fusion of bacterial transcription and translation signals to a complete alfalfa glutamine synthetase coding sequence. Synthesis of the alfalfa glutamine synthetase enzyme in Escherichia coli was demonstrated by functional genetic complementation of a glutamine synthetase-deficient mutant and by immunoblotting analysis. These results should facilitate protein engineering and structure-function analysis of the plant enzyme.