Cultivation of the Drosophila Sex-Ratio Spiroplasma

Science  06 Jun 1986:
Vol. 232, Issue 4755, pp. 1253-1255
DOI: 10.1126/science.232.4755.1253


Uncultivable for more than 25 years, the sex-ratio spiroplasma of Drosophila willistoni grew in a tissue culture medium (H-2) containing an embryo-derived lepidopteran cell line (IPLB-TN-R2). After adaptation, it grew in a cell-free H-2 medium. This success demonstrates the usefulness of cell culture systems for cultivation of fastidious microorganisms and facilitates study of the sex-ratio trait in Drosophila.