Two pairs of recombination signals are sufficient to cause immunoglobulin V-(D)-J joining

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Science  20 Nov 1987:
Vol. 238, Issue 4830, pp. 1134-1138
DOI: 10.1126/science.3120312


The minimum sequence requirements for antigen receptor V-(D)-J joining were studied by constructing recombination-substrates containing synthetic recombination signals and introducing them into a recombination-competent pre-B cell line. Two sets of heptamer (CACTGTG) and nonamer (GGTTTTTGT) sequences were shown to be sufficient to cause the V-(D)-J joining, if the 12- and 23-base pair spacer rule is satisfied. A point mutation in the heptamer sequence, or a change in the combination of the two spacer lengths, drastically reduced the recombination.