Research Articles

Induction of an antibody that catalyzes the hydrolysis of an amide bond

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Science  02 Sep 1988:
Vol. 241, Issue 4870, pp. 1188-1191
DOI: 10.1126/science.3413482


Catalysis of amide bond hydrolysis is of singular importance in enzymology. An antibody was induced to an analog of a high-energy intermediate anticipated along the reaction coordinate of amide hydrolysis. This antibody is an amidase with high specificity and a large rate enhancement (250,000) relative to the uncatalyzed reaction. This reaction represents the kinetically most difficult hydrolysis reaction yet catalyzed by an antibody.