Reports

Prevention of rapid intracellular degradation of ODC by a carboxyl-terminal truncation

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Science  17 Mar 1989:
Vol. 243, Issue 4897, pp. 1493-1495
DOI: 10.1126/science.2928784

Abstract

Ornithine decarboxylase (ODC) was converted from a protein with a short intracellular half-life in mammalian cells to a stable protein by truncating 37 residues at its carboxyl terminus. Cells expressing wild-type protein lost ODC activity with a half-life of approximately 1 hour. Cells expressing the truncated protein, however, retained full activity for at least 4 hours. Pulse-chase experiments in which immunoprecipitation and gel electrophoresis were used confirmed the stabilizing effect of the truncation. Thus, a carboxyl-terminal domain is responsible for the rapid intracellular degradation of murine ODC.