Sequence-specific isotope effects on the cleavage of DNA by bleomycin

Science  22 Sep 1989:
Vol. 245, Issue 4924, pp. 1396-1399
DOI: 10.1126/science.2476851


Bleomycin is a metal- and oxygen-dependent DNA cleaver. The chemistry of DNA damage has been proposed to involve rate-limiting abstraction of the 4'-hydrogen. A DNA fragment has been prepared that contains [4'-2H]thymidine residues of high isotopic content. Primary kinetic isotope effects have been directly observed at individual thymidine residues with DNA sequencing technology.