Articles

Microcolumn separations and the analysis of single cells

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Science  06 Oct 1989:
Vol. 246, Issue 4926, pp. 57-63
DOI: 10.1126/science.2675314

Abstract

Capillary zone electrophoresis and open tubular liquid chromatography are two examples of an emerging area of analytical instrumentation known as microcolumn separations. The high resolution and small sample requirements of these methods make them suitable for the quantitative, multicomponent chemical analysis of single cells. Appropriate instrumentation for the analysis of nanoliter and subnanoliter samples is discussed. Data from the analysis of individual neurons are presented, including amino acid and neurotransmitter content.