Research Articles

Biophysical and molecular mechanisms of Shaker potassium channel inactivation

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Science  26 Oct 1990:
Vol. 250, Issue 4980, pp. 533-538
DOI: 10.1126/science.2122519

Abstract

The potassium channels encoded by the Drosophila Shaker gene activate and inactivate rapidly when the membrane potential becomes more positive. Site-directed mutagenesis and single-channel patch-clamp recording were used to explore the molecular transitions that underlie inactivation in Shaker potassium channels expressed in Xenopus oocytes. A region near the amino terminus with an important role in inactivation has now been identified. The results suggest a model where this region forms a cytoplasmic domain that interacts with the open channel to cause inactivation.