Reports

Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide

Science  06 Dec 1991:
Vol. 254, Issue 5037, pp. 1497-1500
DOI: 10.1126/science.1962210

Abstract

A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement. The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.

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