Reproducible Imaging and Dissection of Plasmid DNA Under Liquid with the Atomic Force Microscope

Science  22 May 1992:
Vol. 256, Issue 5060, pp. 1180-1184
DOI: 10.1126/science.256.5060.1180


Reproducible images of uncoated DNA in the atomic force microscope (AFM) have been obtained by imaging plasmid DNA on mica in n-propanol. Specially sharpened AFM tips give images with reproducible features several nanometers in size along the DNA. Plasmids can be dissected in propanol by increasing the force applied by the AFM tip at selected locations.