Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis

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Science  20 Nov 1992:
Vol. 258, Issue 5086, pp. 1353-1355
DOI: 10.1126/science.1455229


A gene from the flowering plant Arabidopsis thaliana that encodes an omega-3 desaturase was cloned on the basis of the genetic map position of a mutation affecting membrane and storage lipid fatty acid composition. Yeast artificial chromosomes covering the genetic locus were identified and used to probe a seed complementary DNA library. A complementary DNA clone for the desaturase was identified and introduced into roots of both wild-type and mutant plants by Ti plasmid-mediated transformation. Transgenic tissues of both mutant and wild-type plants had significantly increased amounts of the fatty acid produced by this desaturase.