Tumor cell growth arrest caused by subchromosomal transferable DNA fragments from chromosome 11

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Science  16 Apr 1993:
Vol. 260, Issue 5106, pp. 361-364
DOI: 10.1126/science.8469989


A fundamental problem in the identification and isolation of tumor suppressor and other growth-inhibiting genes is the loss of power of genetic complementation at the subchromosomal level. A direct genetic strategy was developed to isolate subchromosomal transferable fragments (STFs) from any chromosome, each containing a selectable marker within the human DNA, that could be transferred to any mammalian cell. As a test of the method, several overlapping STFs from 11p15 were shown to cause in vitro growth arrest of rhabdomyosarcoma cells. This activity mapped between the beta-globin and insulin genes.