Long-range cis preference in DNA homology search over the length of a Drosophila chromosome

Science  18 Mar 1994:
Vol. 263, Issue 5153, pp. 1623-1625
DOI: 10.1126/science.8128250


P element-induced chromosome breakage on the X chromosome of Drosophila melanogaster was repaired six times more frequently when a homologous template was located anywhere on the X chromosome rather than on an autosome. Cis-trans comparisons confirmed that recombinational repair was more frequent when the interacting sequences were physically connected. These results suggest that the search for homology between the broken ends and a matching template sequence occurs preferentially in the cis configuration. This cis advantage operates over more than 15 megabases of DNA.

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