A mass spectrometric solution to the address problem of combinatorial libraries

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Science  15 Apr 1994:
Vol. 264, Issue 5157, pp. 399-402
DOI: 10.1126/science.8153627


The molecular weights of femtomole quantities of small peptides attached to polystyrene beads have been determined with imaging time-of-flight secondary ion mass spectrometry. The analysis is made possible by the selective clipping of the bond linking the peptide to a bead with trifluoroacetic acid vapor before the secondary ion mass spectrometry assay. The approach can be applied to large numbers of 30- to 60-micrometer polystyrene beads for the direct characterization of massive combinatorial libraries.