Direct inhibition of the yeast cyclin-dependent kinase Cdc28-Cln by Far1

Science  26 Aug 1994:
Vol. 265, Issue 5176, pp. 1228-1231
DOI: 10.1126/science.8066461


Cell cycle arrest of Saccharomyces cerevisiae in G1 by the antimitogen alpha-factor is mediated by activation of a signal transduction pathway that results in inhibition of the cyclin-dependent kinase Cdc28-Cln. The Far1 protein is required for cell cycle arrest and associates with the Cdc28-Cln complex. The kinase activity of Cdc28-Cln was directly inhibited by Far1 both in vivo and in vitro, thus demonstrating that Far1 acts at the final step in the alpha-factor response pathway by inhibiting a G1 cyclin-dependent kinase.

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