Requirement of a small cytoplasmic RNA for the establishment of thermotolerance

Science  19 May 1995:
Vol. 268, Issue 5213, pp. 1036-1039
DOI: 10.1126/science.7754381


Thermotolerance is an inducible state that endows cells with an enhanced resistance to thermal killing. Heat shock proteins are believed, and in a few instances have been shown, to be the agents conferring this resistance. The role of a small cytoplasmic RNA (G8 RNA) in developing thermotolerance in Tetrahymena thermophila was investigated by creating a strain devoid of all functional G8 genes. These G8 null cells mounted an apparently normal heat shock response, but they were unable to establish thermotolerance.