Reports

Inducible gene targeting in mice

Science  08 Sep 1995:
Vol. 269, Issue 5229, pp. 1427-1429
DOI: 10.1126/science.7660125

Abstract

A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.

Related Content