Research Articles

Crystal Structure of the MATa1/MATα2 Homeodomain Heterodimer Bound to DNA

Science  13 Oct 1995:
Vol. 270, Issue 5234, pp. 262-269
DOI: 10.1126/science.270.5234.262


The Saccharomyces cerevisiae MATa1 and MATα2 homeodomain proteins, which play a role in determining yeast cell type, form a heterodimer that binds DNA and represses transcription in a cell type-specific manner. Whereas the α2 and a1 proteins on their own have only modest affinity for DNA, the a1/α2 heterodimer binds DNA with high specificity and affinity. The three-dimensional crystal structure of the a1/α2 homeodomain heterodimer bound to DNA was determined at a resolution of 2.5 Å. The a1 and α2 homeo- domains bind in a head-to-tail orientation, with heterodimer contacts mediated by a 16-residue tail located carboxyl-terminal to the α2 homeodomain. This tail becomes ordered in the presence of a1, part of it forming a short amphipathic helix that packs against the a1 homeodomain between helices 1 and 2. A pronounced 60° bend is induced in the DNA, which makes possible protein-protein and protein-DNA contacts that could not take place in a straight DNA fragment. Complex formation mediated by flexible protein-recognition peptides attached to stably folded DNA binding domains may prove to be a general feature of the architecture of other classes of eukaryotic transcriptional regulators.

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