Water and proteins in supercritical CO<sub>2</sub>

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Science  02 Feb 1996:
Vol. 271, Issue 5249, pp. 573
DOI: 10.1126/science.271.5249.573b


Supercritical carbon dioxide (scCO2) can serve as an environmentally friendly replacement solvent in a number of applications, but usually for hydrophobic compounds. Johnston et al. (p. 624; see the Perspective by Beckman, (p. 613;) have found a nontoxic surfactant that stabilizes microdroplets of water in scCO2. Proteins and ionic species can dissolve in these microdroplets, thus allowing these compounds to be processed with scCO2 methods.