Research Article

A Permease-Oxidase Complex Involved in High-Affinity Iron Uptake in Yeast

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Science  15 Mar 1996:
Vol. 271, Issue 5255, pp. 1552-1557
DOI: 10.1126/science.271.5255.1552

Abstract

Iron must cross biological membranes to reach essential intracellular enzymes. Two proteins in the plasma membrane of yeast—a multicopper oxidase, encoded by the FET3 gene, and a permease, encoded by the FTR1 gene—were shown to mediate high-affinity iron uptake. FET3 expression was required for FTR1 protein to be transported to the plasma membrane. FTR1 expression was required for apo-FET3 protein to be loaded with copper and thus acquire oxidase activity. FTR1 protein also played a direct role in iron transport. Mutations in a conserved sequence motif of FTR1 specifically blocked iron transport.

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