Genetically Engineered Resistance to Dengue-2 Virus Transmission in Mosquitoes

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Science  10 May 1996:
Vol. 272, Issue 5263, pp. 884-886
DOI: 10.1126/science.272.5263.884


The control of arthropod-borne virus diseases such as dengue may ultimately require the genetic manipulation of mosquito vectors to disrupt virus transmission to human populations. To reduce the ability of mosquitoes to transmit dengue viruses, a recombinant Sindbis virus was used to transduce female Aedes aegypti with a 567-base antisense RNA targeted to the premembrane coding region of dengue type 2 (DEN-2) virus. The transduced mosquitoes were unable to support replication of DEN-2 virus in their salivary glands and therefore were not able to transmit the virus.