Report

Identification of Subunits of the Anaphase-Promoting Complex of Saccharomyces cerevisiae

Science  15 Nov 1996:
Vol. 274, Issue 5290, pp. 1201-1204
DOI: 10.1126/science.274.5290.1201

Abstract

Entry into anaphase and proteolysis of B-type cyclins depend on a complex containing the tetratricopeptide repeat proteins Cdc16p, Cdc23p, and Cdc27p. This particle, called the anaphase-promoting complex (APC) or cyclosome, functions as a cell cycle-regulated ubiquitin-protein ligase. Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the APC1 gene, is conserved between fungi and vertebrates and shows similarity to BIMEp from Aspergillus nidulans. A small heat-inducible subunit is encoded by the CDC26 gene. The yeast APC is a 36S particle that contains at least seven different proteins.

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