Role for the Amino-Terminal Region of Human TBP in U6 snRNA Transcription

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Science  21 Feb 1997:
Vol. 275, Issue 5303, pp. 1136-1140
DOI: 10.1126/science.275.5303.1136

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Basal transcription from the human RNA polymerase III U6 promoter depends on a TATA box that recruits the TATA box-binding protein (TBP) and a proximal sequence element that recruits the small nuclear RNA (snRNA)-activating protein complex (SNAPc). TBP consists of a conserved carboxyl-terminal domain that performs all known functions of the protein and a nonconserved amino-terminal region of unknown function. Here, the amino-terminal region is shown to down-regulate binding of TBP to the U6 TATA box, mediate cooperative binding with SNAPc to the U6 promoter, and enhance U6 transcription.

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