RNA Polymerase β′ Subunit: A Target of DNA Binding-Independent Activation

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Science  14 Mar 1997:
Vol. 275, Issue 5306, pp. 1655-1657
DOI: 10.1126/science.275.5306.1655

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The bacteriophage N4 single-stranded DNA binding protein (N4SSB) activates transcription by the Escherichia coli σ70-RNA polymerase at N4 late promoters. Here it is shown that the single-stranded DNA binding activity of N4SSB is not required for transcriptional activation. N4SSB interacts with the carboxyl terminus of the RNA polymerase β′ subunit in a region that is highly conserved in the largest subunits of prokaryotic and eukaryotic RNA polymerases.

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