Inducible Expression and Phosphorylation of Coactivator BOB.1/OBF.1 in T Cells

Science  11 Jul 1997:
Vol. 277, Issue 5323, pp. 221-225
DOI: 10.1126/science.277.5323.221

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BOB.1/OBF.1 is a transcriptional coactivator that is constitutively expressed in B cells and interacts with the Oct1 and Oct2 transcription factors. Upon activation of Jurkat T cells and primary murine thymocytes with phorbol esters and ionomycin, BOB.1/OBF.1 expression and transactivation function were induced. BOB.1/OBF.1 was phosphorylated at Ser184 both in vivo and in vitro, and this modification was required for inducible activation. Mutation of Ser184 also diminished transactivation function in B cells, suggesting that the activating phosphorylation that is inducible in T cells is constitutively present in B cells. Thus, BOB.1/OBF.1 is a transcriptional coactivator that is critically regulated by posttranslational modifications to mediate cell type–specific gene expression.

  • * Present address: Novartis Pharma, Preclinical Research, Basel, Switzerland.

  • These authors contributed equally to this work.

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