Report

In Situ Activation Pattern of Drosophila EGF Receptor Pathway During Development

See allHide authors and affiliations

Science  22 Aug 1997:
Vol. 277, Issue 5329, pp. 1103-1106
DOI: 10.1126/science.277.5329.1103

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution

Abstract

Signaling cascades triggered by receptor tyrosine kinases (RTKs) participate in diverse developmental processes. The active state of these signaling pathways was monitored by examination of the in situ distribution of the active, dual phosphorylated form of mitogen-activated protein kinase (ERK) with a specific monoclonal antibody. Detection of the active state of the Drosophilaepidermal growth factor receptor (DER) pathway allowed the visualization of gradients and boundaries of receptor activation, assessment of the distribution of activating ligands, and analysis of interplay with the inhibitory ligand Argos. This in situ approach can be used to monitor other receptor-triggered pathways in a wide range of organisms.

  • * To whom correspondence should be addressed. E-mail: lvshilo{at}weizmann.weizmann.ac.il

View Full Text