Dynamic Molecular Combing: Stretching the Whole Human Genome for High-Resolution Studies

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Science  05 Sep 1997:
Vol. 277, Issue 5331, pp. 1518-1523
DOI: 10.1126/science.277.5331.1518

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DNA in amounts representative of hundreds of eukaryotic genomes was extended on silanized surfaces by dynamic molecular combing. The precise measurement of hybridized DNA probes was achieved directly without requiring normalization. This approach was validated with the high-resolution mapping of cosmid contigs on a yeast artificial chromosome (YAC) within yeast genomic DNA. It was extended to human genomic DNA for precise measurements ranging from 7 to 150 kilobases, of gaps within a contig, and of microdeletions in the tuberous sclerosis 2 gene on patients' DNA. The simplicity, reproducibility, and precision of this approach makes it a powerful tool for a variety of genomic studies.

  • * These authors contributed equally to this work.

  • Present address: INSERM U309, Institut Albert Bonniot, 39706 La Tronche Cedex, France.

  • To whom correspondence should be addressed. E-mail: abensim{at}

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