Report

Regulation of Distinct Stages of Skeletal Muscle Differentiation by Mitogen-Activated Protein Kinases

+ See all authors and affiliations

Science  14 Nov 1997:
Vol. 278, Issue 5341, pp. 1288-1291
DOI: 10.1126/science.278.5341.1288

You are currently viewing the abstract.

View Full Text

Via your Institution

Log in through your institution

Log in through your institution


Abstract

The signal transduction pathway or pathways linking extracellular signals to myogenesis are poorly defined. Upon mitogen withdrawal from C2C12 myoblasts, the mitogen-activated protein kinase (MAPK) p42Erk2 is inactivated concomitant with up-regulation of muscle-specific genes. Overexpression of MAPK phosphatase-1 (MKP-1) inhibited p42Erk2 activity and was sufficient to relieve the inhibitory effects of mitogens on muscle-specific gene expression. Later during myogenesis, endogenous expression of MKP-1 decreased. MKP-1 overexpression during differentiation prevented myotube formation despite appropriate expression of myosin heavy chain. This indicates that muscle-specific gene expression is necessary but not sufficient to commit differentiated myocytes to myotubes and suggests a function for the MAPKs during the early and late stages of skeletal muscle differentiation.

  • * To whom correspondence should be addressed. E-mail: tonks{at}cshl.org

View Full Text

Related Content