Report

Engineering Broader Specificity into an Antibiotic-Producing Polyketide Synthase

See allHide authors and affiliations

Science  09 Jan 1998:
Vol. 279, Issue 5348, pp. 199-202
DOI: 10.1126/science.279.5348.199

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution

Abstract

The wide-specificity loading module for the avermectin-producing polyketide synthase was grafted onto the first multienzyme component (DEBS1) of the erythromycin-producing polyketide synthase in place of the normal loading module. Expression of this hybrid enzyme in the erythromycin producer Saccharopolyspora erythraea produced several novel antibiotic erythromycins derived from endogenous branched-chain acid starter units typical of natural avermectins. Because the avermectin polyketide synthase is known to accept more than 40 alternative carboxylic acids as starter units, this approach opens the way to facile production of novel analogs of antibiotic macrolides.

  • * To whom correspondence should be addressed. E-mail: pfl10{at}mole.bio.cam.ac.uk

View Full Text