Fission Yeast Slp1: An Effector of the Mad2-Dependent Spindle Checkpoint

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Science  13 Feb 1998:
Vol. 279, Issue 5353, pp. 1045-1047
DOI: 10.1126/science.279.5353.1045

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Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan–aspartic acid)–repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.

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