Report

Identification of a Cullin Homology Region in a Subunit of the Anaphase-Promoting Complex

Science  20 Feb 1998:
Vol. 279, Issue 5354, pp. 1219-1222
DOI: 10.1126/science.279.5354.1219

You are currently viewing the abstract.

View Full Text

Via your Institution

Log in through your institution

Log in through your institution


Abstract

The anaphase-promoting complex is composed of eight protein subunits, including BimE (APC1), CDC27 (APC3), CDC16 (APC6), and CDC23 (APC8). The remaining four human APC subunits, APC2, APC4, APC5, and APC7, as well as human CDC23, were cloned. APC7 contains multiple copies of the tetratrico peptide repeat, similar to CDC16, CDC23, and CDC27. Whereas APC4 and APC5 share no similarity to proteins of known function, APC2 contains a region that is similar to a sequence in cullins, a family of proteins implicated in the ubiquitination of G1 phase cyclins and cyclin-dependent kinase inhibitors. The APC2 gene is essential in Saccharomyces cerevisiae, and apc2 mutants arrest at metaphase and are defective in the degradation of Pds1p. APC2 and cullins may be distantly related members of a ubiquitin ligase family that targets cell cycle regulators for degradation.

  • * Present address: Research Institute for Molecular Pathology, Dr. Bohr-Gasse #7, A-1030 Vienna, Austria.

  • To whom correspondence should be addressed. E-mail: marc{at}hms.harvard.edu

View Full Text

Related Content