Coupling Termination of Transcription to Messenger RNA Maturation in Yeast

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Science  10 Apr 1998:
Vol. 280, Issue 5361, pp. 298-301
DOI: 10.1126/science.280.5361.298

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The direct association between messenger RNA (mRNA) 3′-end processing and the termination of transcription was established for theCYC1 gene of Saccharomyces cerevisiae. The mutation of factors involved in the initial cleavage of the primary transcript at the poly(A) site (RNA14, RNA15, andPCF11) disrupted transcription termination at the 3′ end of the CYC1 gene. In contrast, the mutation of factors involved in the subsequent polyadenylation step (PAP1,FIP1, and YTH1) had little effect. Thus, cleavage factors link transcription termination of RNA polymerase II with pre-mRNA 3′-end processing.

  • * Present address: Department of Biological Chemistry, 240 D Med Sci I, College of Medicine, University of California, Irvine, CA 92697, USA.

  • To whom correspondence should be addressed. E-mail: nicholas.proudfoot{at}

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